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In this chapter, we develop a novel architecture for content delivery in the mobile environment (ACME) to address these performance, scalability, and architectural challenges Content delivery techniques have been quite effective in scaling the Internet backbone to accommodate the rapid growth of the World Wide Web traf c In the rst few years of the Web s existence, the Internet backbone (especially ISP peering points) and origin servers were the bottleneck of performance By utilizing Web caches at the edge of the network to store and serve popular Web content, as illustrated in Figure 61a, content delivery techniques reduce the usage of the backbone and signi cantly enhance web performance and user experience However, directly applying wireline content delivery techniques in mobile networks would provide only limited QoS improvement beyond conventional client browser caching.

MARCH 3, 2025 (continued)

Hydrodynamic shockwaves (HDS) are generated either electrically (capacitor discharge system) or by using explosives in water. Besides tenderizing meat products by disrupting the myo brillar structure (Schilling et al. 2003), HDS might in uence bacterial counts as well, resulting in extended product shelf life (Raloff 1998). Explosively produced HDS are not commercially feasible because it is a batch-type process, has speci c packaging requirements, and has potential worker safety concerns. In contrast, electrically generated HDS has been commercialized by Hydrodyne, Inc. (Claus et al. 2001). Mixed results are found in the literature on the effectiveness of HDS to inactivate microbes on meats. Williams-Campbell and Solomon (2002) showed that explosively generated shockwaves caused immediate reduction of aerobic plate counts by 1.5 to 2.0 log10 CFU/g in fresh beef. After 14 days of storage, treated beef counts were 4.5 logs less than control samples. Schilling et al. (2003) showed that blade-tenderized beef treated with HDS had lower standard plate counts (0.5 log difference) compared to controls after 14 days of storage. On the other hand, Moeller et al. (1999) found no signi cant difference in aerobic plate counts and coliform counts between explosive HDS-

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as n . By Lemma 11.6.1, the fact that the relative entropy is small implies that the L1 distance is small, which in turn implies that maxa X |PXn (a) P (a)| is small. Thus, Pr(|PXn (a) P (a)| |PXn E) 0 as n . Alternatively, this can be written as Pr(X1 = a|PXn E) P (a) in probability, a X. (11.171)

In contrast to the Internet backbone bottleneck, the performance bottleneck of mobile Web applications is at the air interface as shown in Figure 61b As a result, user-perceived latency cannot be effectively improved even if content is cached and served from the access network (the edge in traditional content delivery) Recognizing this fundamental difference, we implement the functionality of an edge cache in mobile terminals in ACME, as shown in Figure 62 Edge caching is a superset of client caching and stores popular content requested by all users To implement edge caching functionality at the terminal, ACME pushes content requested by one terminal to other terminals, which cache it for future references Push can be implemented in several different ways One implementation takes advantage of the emerging mobile multicast technologies such as MBMS and DVB-T discussed in 10.

The UserForm s Initialize procedure follows. This procedure creates a Range object named rng that consists of the active sheet s used range. Additional code sets the ListBox s ColumnCount and RowSource properties, and adjusts the ColumnWidths property such that the ListBox columns are proportional to the column widths in the worksheet.

used. The ratio of FuGENE 6 ( l) to DNA ( g) must always be greater than 1. In our own investigations, ratios of 2 5 (v/w) FuGENE 6 to DNA have been successful. Another important consideration is the pericellular matrix that surrounds the chondrocytes, thereby acting as a physical barrier. It is thought to inhibit the uptake of the lipid-DNA complexes into the cell by limiting their interaction with the cell membrane. When this matrix is partially degraded by incubation with hyaluronidase, transfection ef ciencies are signi cantly enhanced [Madry and Trippel, 2000]. Other factors for a successful transfection include the growth state of the cells [Corsaro and Pearson, 1981] and an optimal cell density at the time of transfection. It is therefore important to maintain a standard seeding protocol. The protocol described in Section 3 has proven successful for the authors in transfecting neonatal bovine and human articular chondrocytes. It may serve as a starting point for further improvements, as transfection conditions vary among species, as well as with the anatomic location and the age of the donor. Although a list of reagents is included with the protocols, similar results may be obtained with reagents from different manufacturers. When chondrocytes from a different species (e.g., avian chondrocytes) are used, transfections with the commercially available Lipofectin or Lipofectamine may be considered. Alternatively, cationic liposomes may be synthesized from their individual components [Ravid and Freshney, 1998].

9

The FCC regulation states that succeeding communications to E911 should be located with an accuracy of 50 m 67% of the time and 150 m 95% of the time. 2 Directive 2002/22 on E112.