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The MIDC (Immelman, 1999, 2005) is also a new instrument, but it has already demonstrated its promise as an indirect rating measure of the personality characteristics of national presidents (Immelman, 2002) as well as presidential candidates (Immelman, 1998) The MIPS has been found useful for employment screening and personnel selection (T Millon, 1994; Weiss, 2002), assessing the personality characteristics of Navy divers (Beckman, Lall, & Johnson, 1996), and in identifying traits associated with different relaxation styles (Sohnle, 2001) as well as the theoretical preferences of counseling students (Scragg, Bor, & Watts, 1999) The MIPS Adjustment Index has been empirically demonstrated to identify individuals with poor work performance and those who may need psychological help (T Millon, 1994; Weiss, 2002).

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PACL scales have been used to study occupational preferences (Plante & Boccaccini, 1997; Strack, 1994), the characteristics of competitive athletes (Gat & McWhirter, 1998), family systems (Gontag & Erickson, 1996; Horton & Retzlaff, 1991), individual differences in social judgment (Moore, Smith, & Gonzalez, 1997), interpersonal problems (Pincus & Wiggins, 1990), and outcomes among elderly veterans with posttraumatic stress disorder (Hyer & Boyd, 1996) The PACL Problem Indicator scale.

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Growth medium (See Section 27) PBSA Trypsin-EDTA: trypsin, 025%, EDTA, 1 mM in PBSA Plastic centrifuge tube, 50 ml (eg, Falcon) Protocol (a) Resuspend the chondrocytes in growth medium by gentle trituration The volume of medium will depend on the starting cell number and the required cell concentration for the experiment For example, if chondrocytes are to be seeded in 75 cm2 asks (20 ml/ ask) at an initial density of 25 104 cells/cm2 , each ask will require 1875 106 cells, suspended in 20 ml of culture medium Periodic resuspension of the chondrocytes is recommended to keep the cell density consistent (b) Maintain cultures at 37 C in a 95% air-5% CO2 humidi ed incubator (c) Refeed the cells with fresh growth medium every 2 3 days, as required, until con uence.

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3531 Mapping the CAF to IMS The Context Awareness Function can be integrated into the IMS as a speci c Application Server providing context information including presence and location This implies that the CAF could perform functions of a Presence Server and a Location Server In this case the CAF would replace or complement the existing Presence and Location Server The CAF has to work closely with the Serving Call State Control Function (S-CSCF) and the Home Subscriber Server (HSS) providing user location (in terms of access network), subscription, policies, etc Furthermore, the HSS and S-CSCF both can be regarded as context providers for the CAF The HSS is the main data storage for all IMS subscribers and service-related data within an IMS domain.

The time required to reach con uence varies considerably, depending on donor age A minimum of 4 days would be expected for a fourfold increase in cell density (d) At con uence, aspirate the medium and rinse the cell layer with PBSA (e) Add approximately 10 ml trypsin/EDTA per 20 cm2 Tip the ask to distribute the trypsin-EDTA across the entire surface of the cell layer and incubate at 37 C for 5 10 min, or until the cells begin to detach (f) Dislodge the chondrocytes from the surface by lightly tapping the bottom of the ask against a hard surface (g) Add 10 volumes of growth medium and complete the cell dislodgement and resuspension by vigorous pipetting (h) Transfer the suspension to a Falcon tube Rinse the ask with 3 ml medium and transfer the rinse to the Falcon tube.

(i) Pellet the cells by centrifugation at 250 g for 5 min (j) Resuspend the chondrocytes in fresh culture medium and count the cells with a hemocytometer (k) In some instances, chondrocyte monolayers detach as a single sheet and do not dissociate into single cells Secondary digestion of the chondrocyte layer in 005% collagenase A for 1 2 h, similar to the protocol provided for primary chondrocyte isolation (Protocol 41), provides single-cell suspensions (l) Reseed the passaged chondrocytes into asks at the appropriate density..

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There are many others but most of them are just copycats that do not know anything about barcode standards. PS: Do not use a font for ...
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